HP - LC

It produces exact volume of metabolite available in the body and interpretation can be given that since how long it had been consumed. The quantity depends on the amount of nicotine in the Cigarette and the manner in which a person smoke, even as how deeply they inhale. It evaluates the exact graph of rise of risks carried by the smoker.

Quantitative testing BY ELISA, HIGH PERFOMANCE-LIQUID CHROMATOGRAPHY & LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY METHOD can help distinguish between passive smoker & active smoker, tobacco user who have recently quit, non tobacco users who have been exposed to significant environmental tobacco smoke and non user who have not been exposed.

TEST PRINCIPLE-ELISA

The Cotinine ELISA is an immunoassay for the quantitative and sensitive detection of Cotinine. This test is suitable for the quantitative and/or qualitative screening of Cotinine in water, urine, and saliva samples. The test is a direct competitive ELISA which is based on the recognition of Cotinine by specific antibodies. Cotinine, when present in a sample and a Cotinine-HRP analogue compete for the binding sites of rabbit anti-Cotinine antibodies in solution. The Cotinine antibodies are then bound by as second antibody(goat anti- rabbit) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Cotinine present in the sample. The color reaction is stopped after as specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

TEST PRINCIPLE-HPLC :

HPLC is an analytical technique to understand the elemental composition of a drug substance, chemical substance, or food product. It is used to separate the components in a mixture, to identify each component, and to quantify each component. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.