Nicotine detection using urine is very sensitive and will detect even small levels of second-hand smoke. It is the most common method used by insurance companies to differentiate active smokers from passive smokers. Whether nicotine detection is through urine, saliva or blood serum, the difference can be seen between people who actively smoke three or more cigarettes a day and those with high levels of second-hand smoke. Heavy smokers can also be differentiated from light smokers by the amount of Cotinine in the sample.